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Test Code IABCS B-Cell Phenotyping Profile for Immunodeficiency and Immune Competence Assessment, Blood

Reporting Name

Immune Assessment B Cell Subsets, B

Useful For

Screening for common variable immunodeficiency (CVID) and hyper-IgM syndromes

 

Assessing B-cell subset reconstitution after stem cell or bone marrow transplant

 

Assessing response to B-cell-depleting immunotherapy

 

Identifying defects in transmembrane activator and calcium modulator and cyclophilin ligand (CAML) interactor (TACI) and B-cell-activating factor receptor (BAFF-R) in patients presenting with clinical symptoms and other laboratory features consistent with CVID

Profile Information

Test ID Reporting Name Available Separately Always Performed
TBBS QN Lymphocyte Subsets: T, B, and NK Yes Yes
IABC Immune Assessment B Cell Subsets, B No Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
CVID CVID Confirmation Flow Panel Yes No

Testing Algorithm

If immune assessment B-cell subsets test is abnormal, then confirmation will be performed at an additional charge.

 

When multiple specimen types are required to perform a panel of tests, the laboratory will perform the tests for which the appropriate specimen type was received and the laboratory will cancel those for which the appropriate specimen was not received. Please be advised that this may change the degree of interpretation received with the report. If only the refrigerate EDTA sample is received, this test will be canceled and converted to RBCS / Relative B-Cell Subset Analysis Percentage which provides the relative B-cell subset values without quantitation.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Whole Blood EDTA

Specimen Required

Two separate EDTA specimens are required: 1 refrigerated and 1 at ambient transport temperature.

 

For serial monitoring, we recommend that specimen draws be performed at the same time of day.

 

Specimen Type: Whole blood for TBBS / Quantitative Lymphocyte Subsets: T, B, and NK

Container/Tube: 4 mL Lavender top (EDTA)

Specimen Volume: 3 mL

Collection Instructions:

1. Send specimen in original tube. Do not aliquot.

2. Label specimen as blood for TBBS / Quantitative Lymphocyte Subsets: T, B, and NK.

Specimen Stability Information: Ambient <52 hours

 

Specimen Type: Whole blood for IABC / B-Cell Phenotyping Screen for Immunodeficiency and Immune Competence Assessment, Blood

Container/Tube: Lavender top (EDTA)

Specimen Volume:

≤14 years: 4 mL

>14 years: 10 mL

Collection Instructions:

1. Send specimen in original tube. Do not aliquot.

2. Label specimen as blood for IABC / B-Cell Phenotyping Screen for Immunodeficiency and Immune Competence Assessment, Blood.

Specimen Stability Information: Refrigerated <48 hours

Specimen Minimum Volume

TBBS: 1 mL; IABC ≤14 years: 3 mL, >14 years: 5 mL

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood EDTA Varies 48 hours

Reference Values

The appropriate age-related reference values will be provided on the report.

Day(s) and Time(s) Performed

Monday through Friday

Specimens are required to be received in the laboratory on weekdays and by 4 p.m. on Friday. No weekend processing.

CPT Code Information

T- and B-Cell Quantitation by Flow Cytometry

86355-B cells, total count

86357-Natural killer (NK) cells, total count

86359-T cells, total count

86360-Absolute CD4/CD8 count with ratio

 

B-Cell Phenotyping Screen for Immunodeficiency and Immune Competence Assessment, Blood

88184-Flow cytometry, first marker

88185 x 7-Flow cytometry, each additional marker

 

Common Variable Immunodeficiency Confirmation Flow Panel

88184-Flow cytometry, first marker (if appropriate)

88185 x 2-Flow cytometry, each additional marker (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
IABCS Immune Assessment B Cell Subsets, B In Process

 

Result ID Test Result Name Result LOINC Value
30296 CD19+ % of total Lymphocytes 8117-4
3321 CD45 Total Lymph Count 27071-0
3316 % CD3 (T Cells) 8124-0
29094 CD20+ % of total Lymphocytes 8119-0
3322 CD3 (T Cells) 8122-4
30298 CD27+ % of CD19+ B cells In Process
3319 % CD4 (T Cells) 8123-2
30300 CD27+ IgM+ IgD+ % of CD19+ B cells In Process
3325 CD4 (T Cells) 24467-3
30302 CD27+ IgM- IgD- % of CD19+ B cells In Process
3320 % CD8 (T Cells) 8101-8
30304 CD27+ IgM+ IgD- % of CD19+ B cells In Process
3326 CD8 (T Cells) 14135-8
30306 IgM+ % of CD19+ B cells In Process
3318 % CD19 (B Cells) 8117-4
30308 CD38+ IgM- % of CD19+ B cells In Process
30310 CD38+ IgM+ % of CD19+ B cells In Process
3324 CD19 (B Cells) 8116-6
4054 % CD16+CD56 (NK cells) 18267-5
30312 CD21+ % of CD19+ B cells In Process
30314 CD21- % of CD19+ B cells In Process
4055 CD16+CD56 (NK cells) 20402-4
3327 4/8 Ratio 54218-3
30297 CD19+ 8116-6
29095 CD20+ 9558-8
6657 Comment 48767-8
30299 CD27+ In Process
30301 CD27+ IgM+ IgD+ In Process
30303 CD27+ IgM- IgD- In Process
30305 CD27+ IgM+ IgD- In Process
30307 IgM+ In Process
30309 CD38+ IgM- In Process
30311 CD38+ IgM+ In Process
30313 CD21+ 25164-5
30315 CD21- In Process
30316 Interpretation 69048-7

Method Name

TBBS: Flow Cytometry

IABC: Fluorescent Flow Cytometry

Necessary Information

1. Date of draw is required.

2. Ordering physician's name and phone number are required.

Shipping Instructions

Specimens are required to be received in the laboratory weekdays and by 4 p.m. on Friday. It is recommended that specimens arrive within 24 hours of draw. Specimens arriving on the weekend may be canceled. Draw and package specimen as close to shipping time as possible.

Test Classification

See Individual Test IDs