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Test Code IDH IDH1/2, Mutation Analysis

Useful For

Supporting a morphological diagnosis of a diffuse glioma


Assisting in central nervous system tumor classification


Stratifying prognosis of diffuse gliomas


Assessing for the following mutations: IDH1 mutations R132H, R132C, R132G, R132L, R132S, R132P; and IDH2 R172K, R172G, R172M

Additional Tests

Test ID Reporting Name Available Separately Always Performed
SLIRV Slide Review in MG No, (Bill Only) Yes

Testing Algorithm

This test is performed in conjunction with SLIRV / Slide Review. Additional testing may be performed after review by pathologist. Upon approval from the requesting clinician, PATHC / Pathology Consultation may be added, if determined to be appropriate.

Special Instructions

Method Name

Polymerase Chain Reaction (PCR) and Pyrosequencing

Reporting Name

IDH1/2, Mutation Analysis

Specimen Type


Necessary Information

A pathology/diagnostic report including a brief history is required.

If available, include IDH1 mutation (R132H) immunostain results.

Specimen Required

Supplies: Pathology Packaging Kit (T554)

Specimen Type:

Container/Tube: Surgical Pathology Packaging Kit (T554) requested, but not required

Preferred: Formalin-fixed, paraffin-embedded (FFPE) tissue block with a minimum of 40% tumor cell population

Alternate: Unstained slides with a minimum of 40% tumor population; slides may be stained and/or scraped

Collection Instructions:

1. Process all fresh or frozen specimens into FFPE blocks prior to submission.

2. If submitting slides, a minimum of five, 4- to 5-micron thick, unstained slides are required.

Additional Information:

1. A quality specimen is essential for evaluation. Submit only tissue containing tumor cells; minimal tissue is required for evaluation.

2. Special stains performed outside Mayo Medical Laboratories and included with the case may be repeated and charged at the reviewing pathologist's discretion. Testing requested by referring physician may not be performed if deemed unnecessary by Mayo Clinic pathologist.

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)

Reference Values

An interpretative report will be provided.

Day(s) and Time(s) Performed

Monday through Friday; Varies

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

81120-IDH1 (isocitrate dehydrogenase 1 [NADP+], soluble) (eg, glioma), common exon 4 variants (eg, R132H, R132C)

81121-IDH2 (isocitrate dehydrogenase 2 [NADP+], mitochondrial) (eg, glioma), common exon 4 variants (eg, R140W, R172M)

88381-Microdissection, manual

LOINC Code Information

Test ID Test Order Name Order LOINC Value
IDH IDH1/2, Mutation Analysis In Process


Result ID Test Result Name Result LOINC Value
52948 Result Summary 50397-9
52949 Result In Process
52950 Interpretation 50595-8
52951 Additional Information 48767-8
52952 Reason for Referral 42349-1
52953 Specimen 31208-2
52954 Source 31208-2
52955 Tissue ID No LOINC Needed
52956 Released By No LOINC Needed


1. Pathology/Cytology Information (T707) in Special Instructions

2. If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

Pathology Test Request Form (T246) (

Oncology Test Request Form (T729) (