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Test Code LMALP Malaria PCR with Parasitemia Reflex

Useful For

Detection of Plasmodium DNA and identification of the infecting species

 

An adjunct to conventional microscopy of Giemsa-stained films

 

Detection and confirmatory identification of Plasmodium species: Plasmodium falciparum, Plasmodium vivax, Plasmodium ovale, Plasmodium malariae, and Plasmodium knowlesi

Testing Algorithm

If positive, PARCT / Malaria Percent Parasitemia will be performed at an additional charge.

 

See Malaria Laboratory Testing Algorithm in Special Instructions.

Method Name

Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

Reporting Name

Malaria PCR with Parasitemia Reflex

Specimen Type

Varies

Specimen Required

Forms: If not ordering electronically, complete, print, and send a Microbiology Request Form (T244) with the specimen (www.mayomedicallaboratories.com/media/customer-service/forms/microbiology-request-form.pdf).

 

Blood and slides are required.

 

Specimen Type: Blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: 3 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Do not transfer blood to other containers. Send specimen in original tube.

Additional Information:

1. This test is not performed on a STAT basis and, therefore, should not be used as a primary screening test for malaria.

2. This test is used primarily for confirmation of a presumptive malaria diagnosis and determination of infecting Plasmodium species–particularly when the parasite morphology on traditional blood films is suboptimal.

3. Clients in the Rochester, MN area who are seeking a primary test for malaria and who can deliver the specimen within 4 hours of collection should order the MAL / Malaria/Babesia Smear.

4. Laboratories that are unable to deliver a specimen within 4 hours of collection should perform an initial screen for malaria and other blood parasites in their laboratory prior to sending a specimen to Mayo Medical Laboratories.

 

Specimen Type: Blood films

Container/Tube: Clean, grease-free slides in plastic slide container

Specimen Volume: 2 thin blood films and 2 thick blood films

Collection Instructions:

1. Blood films should be made from fresh blood using fingerstick or drops of blood from needle following venipuncture. However, EDTA anticoagulated blood is also acceptable. 

2. Prepare thin blood films as follows:

a. Prepare a thin film with a "feathered edge" that is no more than a single cell thick.

b. Allow the film to thoroughly air dry and then fix by briefly immersing in either absolute or 95% methyl alcohol.

c. Allow to air dry after fixation.

3. Prepare thick blood films as follows:

a. Place a large drop of blood (approximately the size of a dime and preferably from a fingerstick) on a slide.

b. Using a corner of a second slide, spread the drop in a circular motion while applying firm pressure to literally scratch the blood onto the carrier slide. This technique allows the blood to dry quickly and adhere well to the slide. Use approximately 20 circular sweeps with the second slide. The drop of blood should be about the size of a quarter when finished.

c. Do not fix. Air dry thoroughly (approximately 45 minutes) before placing in transport container.

Specimen Minimum Volume

1 mL/Slides: NA

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated (preferred) 7 days
  Ambient  7 days

Reference Values

Negative

If positive, percent parasitemia will be calculated and reported.

Day(s) and Time(s) Performed

Monday through Sunday; Varies

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LMALP Malaria PCR with Parasitemia Reflex In Process

 

Result ID Test Result Name Result LOINC Value
37116 Malaria PCR w/ Parasitemia In Process

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
PARCT Malaria Percent Parasitemia No No