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Test Code RBCME Red Blood Cell Membrane Evaluation, Blood

Useful For

Investigation of suspected red cell membrane disorders such as hereditary spherocytosis or hereditary pyropoikilocytosis

Profile Information

Test ID Reporting Name Available Separately Always Performed
FRAGO Osmotic Fragility Yes, (Order FRAG) Yes
SCTRL Shipping Control Vial No Yes
HSEV Spherocytosis Interpretation No Yes
BND3 Band 3 Fluorescence Staining, RBC No Yes
SMPB Peripheral Blood Smear Review No Yes

Testing Algorithm

Osmotic fragility and eosin-5-maleimide (EMA) binding (Band3) flow cytometry testing will always be performed. A normal shipping control is necessary to exclude false-positive results due to preanalytical artifact. Testing will be canceled if no shipping control is received or if the shipping control is abnormal. A consultative interpretation will be provided.

Method Name

HSEV: Consultative Interpretation

FRAGO: Osmotic Lysis

BND3: Flow Cytometry

SMPB: Consultant Review

Reporting Name

RBC Membrane Evaluation, B

Specimen Type

Control
Whole Blood EDTA
Whole Blood Slide

Specimen Required

A whole blood EDTA specimen, an EDTA control specimen, and 2 well-made peripheral blood smears (Wright stained or fixed in absolute methanol) are required for testing.

 

Patient:

Specimen Type: Blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: 4 mL

Collection Instructions:

1. Immediately refrigerate specimen after draw. Refrigerate at 0 to 4° C. Do not freeze. Freezing causes sample lysis, and tests will not be performed on hemolyzed specimens.

2. Send specimen in original tube. Do not aliquot.

3. Rubber band patient specimen and control vial together. Control must accompany the patient sample at all times to ensure the reliability of testing results.

4. Be sure specimen and control are stored and transported together at refrigerate temperature, carefully following proper handling and shipping instructions.

 

Patient:

Specimen Type: Slides

Container/Tube: Blood smears

Specimen Volume: 2 well-made peripheral blood smears

Collection Instructions: Collect 2 well-made peripheral blood smears (Wright stained or fixed in absolute methanol).

 

Normal Shipping Control:

Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: 4 mL

Collection Instructions:

1. Draw a control specimen from a normal (healthy), unrelated, nonsmoking person at the same time as the patient.

2. Label clearly on outermost label normal control.

3. Immediately refrigerate specimen after draw. Refrigerate at 0 to 4° C. Do not freeze. Freezing causes sample lysis, and tests will not be performed on hemolyzed specimens.

4. Send specimen in original tube. Do not aliquot.

5. Rubber band patient specimen and control vial together. Control must accompany the patient sample at all times to ensure the reliability of testing results.

Specimen Minimum Volume

2 mL

Specimen Stability Information

Specimen Type Temperature Time
Control Refrigerated 72 hours
Whole Blood EDTA Refrigerated 72 hours
Whole Blood Slide Refrigerated

Reference Values

≥12 months:

0.50 g/dL NaCl (unincubated): 3-53 % hemolysis

0.60 g/dL NaCl (incubated): 14-74 % hemolysis

0.65 g/dL NaCl (incubated): 4-40 % hemolysis

0.75 g/dL NaCl (incubated): 1-11 % hemolysis 

An interpretive report will be provided.

 

Reference values have not been established for patients who are <12 months of age. 

Day(s) and Time(s) Performed

Monday through Saturday

Performing Laboratory

Mayo Medical Laboratories in Rochester

CPT Code Information

85557-Osmotic fragility

88184-Flow cytometry; first cell surface, cytoplasmic or nuclear marker x 1

85060-Morphology review

LOINC Code Information

Test ID Test Order Name Order LOINC Value
RBCME RBC Membrane Evaluation, B In Process

 

Result ID Test Result Name Result LOINC Value
83141 Band 3 Fluorescence Staining, RBC In Process
9064 Osmotic Fragility, RBC 34964-7
SCTRL Shipping Control Vial 40431-9
13065 Spherocytosis Interpretation 59466-3
37406 Peripheral Blood Smear Review 59465-5
37436 Reviewed By No LOINC Needed
3306 Osmotic Fragility, 0.50 g/dL NaCl 23915-2
3307 Osmotic Fragility, 0.60 g/dL NaCl 23917-8
3308 Osmotic Fragility, 0.65 g/dL NaCl 23919-4
3309 Osmotic Fragility, 0.75 g/dL NaCl 30543-3
3310 Osmotic Fragility Comment 59466-3

Forms

1. Red Cell Membrane Disorders Information (T706) in Special Instruction

2. If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/benign-hematology-test-request-form.pdf)

Shipping Instructions

Specimens must arrive within 72 hours of draw.

Test Classification

See Individual Test IDs