Sign in →

Test Code TLYMF T-Cell Lymphoma, FISH, Tissue

Useful For

Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with various T-cell lymphomas

 

Tracking known chromosome abnormalities and response to therapy in patients with T-cell lymphomas

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
_PBCT Probe, +2 No, No (Bill Only) No
_PADD Probe, +1 No, No (Bill Only) No
_PB02 Probe, +2 No, No (Bill Only) No
_PB03 Probe, +3 No, No (Bill Only) No
_IL25 Interphases, <25 No, No (Bill Only) No
_I099 Interphases, 25-99 No, No (Bill Only) No
_I300 Interphases, ≥100 No, No (Bill Only) No

Testing Algorithm

This test does not include a pathology consultation. If a pathology consultation is requested, PATHC / Pathology Consultation should be ordered and the appropriate FISH test will be ordered and performed at an additional charge. Mayo Hematopathology Consultants are involved in both the pre-analytic (tissue adequacy and probe selection, when applicable) and post-analytic (interpretation of FISH results in context of specific case, when applicable) phases.

 

This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results.

 

Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.

 

Depending on the lymphoma subtype suspected, the most appropriate probes to order are listed in the Clinical Information.

 

If the patient is being tracked for known abnormalities, indicate which probes should be used.

 

Panel includes testing for the following abnormalities using the probes listed:

14q32.1 rearrangement, TCL1A

-7/7q-/i(7q), D7S486/D7Z1

+8, D8Z2/MYC

2p23.2 rearrangement, ALK

 

This assay detects chromosome abnormalities observed in paraffin-embedded tissue samples of patients with T-cell lymphoma. For testing the blood and bone marrow from patients with T-cell lymphoma, see TLPF / T-Cell Lymphoma, FISH, Blood or Bone Marrow.

Method Name

Fluorescence In Situ Hybridization (FISH)

Reporting Name

T-cell Lymphoma, FISH, Ts

Specimen Type

Tissue


Advisory Information


This test is not appropriate for testing blood or bone marrow samples from patients with T-cell lymphoma, see TLPF / T-Cell Lymphoma, FISH, Blood or Bone Marrow.



Shipping Instructions


Advise Express Mail or equivalent if not on courier service.



Necessary Information


Provide a reason for referral and pathology report with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Lymph node

Preferred: Tissue block

Collection Instructions: Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used

Acceptable: Slides

Collection Instructions: For each probe set ordered, 2 consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.

 

Specimen Type: Solid tumor

Preferred: Tissue block

Collection Instructions: Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used.

Acceptable: Slides

Collection Instructions: For each probe set ordered, 2 consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.


Specimen Minimum Volume

For each probe set ordered, 2 consecutive, unstained, 5 micron-thick sections placed on positively charged slides. Include 1 hematoxylin and eosin-stained slide.

Specimen Stability Information

Specimen Type Temperature Time
Tissue Ambient (preferred)
  Refrigerated 

Reference Values

An interpretive report will be provided.

Day(s) and Time(s) Performed

Samples processed Monday through Sunday. Results reported Monday through Friday 8 a.m.-5 p.m.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

88271x2, 88291 – DNA probe, each (first probe set), Interpretation and report

88271x2 – DNA probe, each; each additional probe set (if appropriate)

88271x1 – DNA probe, each; coverage for sets containing 3 probes (if appropriate)

88271x2 – DNA probe, each; coverage for sets containing 4 probes (if appropriate)

88271x3 – DNA probe, each; coverage for sets containing 5 probes (if appropriate)

88274 w/modifier 52 – Interphase in situ hybridization, <25 cells, each probe set (if appropriate)

88274 – Interphase in situ hybridization, 25 to 99 cells, each probe set (if appropriate)     

88275 – Interphase in situ hybridization, 100 to 300 cells, each probe set (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TLYMF T-cell Lymphoma, FISH, Ts In Process

 

Result ID Test Result Name Result LOINC Value
52139 Result Summary 50397-9
52141 Interpretation In Process
52140 Result Table No LOINC Needed
54583 Result In Process
CG743 Reason for Referral 42349-1
52142 Specimen In Process
52143 Source 31208-2
52144 Tissue ID No LOINC Needed
52145 Method 49549-9
55025 Additional Information 48767-8
53832 Disclaimer 62364-5
52146 Released By No LOINC Needed

Forms

If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request Form (T726) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/hematopathology-request-form.pdf)