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Test Code XUREPC Ureaplasma species, Molecular Detection, PCR

Additional Codes

XUREPC (MWHC)

URRP (MAYO)

Reporting Name

Ureaplasma PCR

Useful For

Rapid, sensitive, and specific identification of Ureaplasma urealyticum and U parvum from genitourinary, reproductive, bone and joint, and lower respiratory sources

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Varies

Specimen Required

The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Ureaplasma DNA is not likely.

 

Submit only 1 of the following specimens:

 

Supplies: M5 Media (T484), Culturette (BBL Culture Swab) (T092)

Specimen Type: Swab

Sources: Vaginal, cervix, urethra, urogenital, chest/mediastinal, bronchus (donor swab), upper respiratory sources (only infants <3 months: nasopharynx, nose, throat)

Container/Tube:

Preferred: Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium [T092])

Acceptable: Swab in transport media: M4, M5 (T484), M6, universal transport media, or ESwab

Specimen Volume: 1 swab

Collection Instructions:

Vaginal:

1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.

2. Place swab back into swab cylinder.

Urethra or Cervical:

1. Collect specimen by inserting swab 1 to 3 cm and rotating 360 degrees.

2. Place swab back into swab cylinder.

Wound:

1. Collect specimen by swabbing back and forth over wound surface to maximize recovery of cells.

2. Place swab back into swab cylinder.

 

Supplies: M5 (T484) media

Specimen Type: Fluid

Sources: Pelvic fluid, amniotic fluid, prostatic secretions, semen, reproductive drainage or fluid, synovial fluid, pleural/chest fluid, chest tube fluid, pericardial fluid, sputum, tracheal secretions, bronchial washings, bronchoalveolar lavage, lung fluid; nasal washings (only infants <3 months)

Container/Tube:

Preferred: Sterile container

Acceptable: Specimen in 3 mL of transport media: M4, M5 (T484), M6, or universal transport media

Specimen Volume: 1-2 mL

 

Specimen Type: Urine, kidney/bladder stone, ureter

Container/Tube: Sterile container

Specimen Volume: 10 mL or entire specimen

 

Specimen Type: Tissue

Sources: Placenta, products of conception, respiratory, bronchus, chest/mediastinal, bone, joint

Container/Tube: Sterile container

Specimen Volume: 5 mm(3)

Collection Instructions: Submit only fresh tissue.

Specimen Minimum Volume

Fluid: 1 mL; Urine: 2 mL; Swab: 1 swab; Tissue: 5 mm(3)

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Not applicable

Day(s) and Time(s) Performed

Monday through Friday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87798 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
URRP Ureaplasma PCR In Process

 

Result ID Test Result Name Result LOINC Value
SRC80 Specimen source 31208-2
35128 Ureaplasma urealyticum PCR 51988-4
35129 Ureaplasma parvum PCR 69933-0

Method Name

Real-Time Polymerase Chain Reaction (PCR) Using LightCycler and Fluorescent Resonance Energy Transfer (FRET)

(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf).

Necessary Information

Specimen source is required.